Growth Factor-Collagen Relationship in Wound Healing

Barbaros Balabanlı, Şule Cevher, Fatma Küçük
2.269 615

Abstract


Various growth factors such as EGF are effective in wound healing. For this reason, it was targeted in our study to observe the impact of systematically given EGF on collagen and protein synthesis in hepatic tissue. Rats to which dorsolateral excisional wound was made were divided into 2 equal groups: 1-Untreated control group, 2-Systemic EGF applied group. A daily dose of EGF (10 ng/ml) was given to the animals in the application group. Rats were sacrificed in 1st, 5th, 7th and 14th days of the study. Collagen and protein concentrations in hepatic tissues were measured spectrophometrically. In consideration of the phases of systemic EGF application and its comparison with the controls, it was determined that EGF stimulates the synthesis of collagen up to 7 days and protein up to 5 days in liver and this stimulation stops in the later days and EGF begins to make pressure on collagen and protein synthesis.


Keywords


Epidermal growth factor, wound healing, collagen, protein synthesis

Full Text:

PDF

References


Diegelmann, R.F. and Evans, M.C., ‘‘Wound

healing: an overview of acute, fibrotic and delayed

healing’’, Front. Biosci., 9: 283-289, (2004).

Engin, A., Yara İyileşmesi: Temel Cerrahi, Sayek,

İ. (ed.), Güneş Kitabevi, Ankara, 266-277, (2004).

Steed, D.L., ‘‘Modifiying the wound healing

response with exogenous growth factors’’, Clin.

Plas. Surg., 25: 397-405, (1998).

Gönül, B., Güvendik, G., Erbaş, D., Yanıçoglu, L.,

‘‘Denerve farelerde epidermal büyüme faktörünün

serum çinko düzeylerine etkisi’’, Ankara Ecz. Fak.

Der., 18: 1-7, (1988).

Cohen, S., ‘‘The epidermal growth factor (EGF)’’,

Cancer, 51: 1787- 1791, (1983).

Erbaş, D., Oygür, T. and Anıl, A., ‘‘Submandibuler

bez ekstresinin yara iyileşmesine olan etkisi’’,

G.Ü. Dişhek. Fak. Derg., 5(2): 139-147, (1988).

Şimşek, M.B., ‘‘Farklı formlardaki epidermci

büyüme faktörünün yara iyileşmesi üzerine

etkisinin histopatolojik olarak araştırılması’’,

Doktora Tezi, Gazi Üniversitesi Sağlık Bilimleri

Enstitüsü, Ankara, 23-42, (1998).

Wingren, U., Franzen, L., Larson, G.M., Malcherek,

P. and Schultz, G.S., ‘‘Epidermal growth factor

accelerates connective tissue wound healing in the

perforaled rat mesentary’’, J. Surg. Res., 53(1): 48- 54, (1992).

Sağlam, M., Bağ dokusu, Genel Histoloji 3 üncü

baskı, Emel Yayıncılık, Ankara, 127-151, (1987).

Gözükara, E.M., Biyokimya, Nobel Tıp

Kitabevleri, Ankara, 152-171, (2001).

Brunicardi, F.C., Andersen, D. A., Billiar, T.R., Dunn, D. L., Hunter, J.G., Matthews, J.B., Pollock, R.E. and Schwartz, I.S., ‘‘Wound healing’’, Schwartz’s Principles of Surgery, The McGraw-Hill Companies, 310-325, (2005).

Calvin, M., ‘‘Cutaneous wound healing’’, Wounds, 1: 12-32, (1998).

Rigler, D.J., Inflamation and Repair 6 th ed., (ed.) Jones, T.C., Hunt, R.D., King, N.W., Veterinary Pathology, Pennsylvania, 150-157, (1997).

Stashak, T.S., ‘‘Principles of wound healing’’, Equine Wound Management, Pennsylvania, 1- 15, (1991).

Deodhar, A.K. and Rana, R.E., ‘‘Surgical physiology of wound healing: a review’’, J. Postgrad. Med., 43(2): 52-56, (1997).

Regan, M.C. and Barbul, A., ‘‘The cellular biology of wound healing’’, Schlag, G., Redl, H. (Ed.), Wound Healing, Germany, 1: 3-17, (1994).

Heinze, C.D. and Clem, M.F., Wound healing and tissue repair 2 nd ed., Oehme, F.W., Textbook of Large Animal Surgery, USA, 141-153, (1988).

Swaim, S.F. and Henderson, R.A., ‘‘Wound healing’’, Small Animal Wound Management, Pennsylvania, 1-8, (1990).

Hedlund, C.S., Surgey of the Integumentary System 2 nd ed., Small Animal Surgery, Fossum, T.W. (Ed.), China, 134-137, (2002).

Theoret, C.L., ‘‘Update on wound repair’’, Clin.

Tech. Equin. Pract., 3: 110-122, (2004).

Broughton, G., Janis, J.E. and Attinger, C.E., ‘‘Wound healing: an overview’’, Plast. Reconstr. Surg., 117: 1-32, (2006).

Lowry, O.H., ‘‘Protein measurement with the folin phenol reagent’’, J. Biol. Chem., 183: 265-275, (1951).

Komsa-Penkova, R., Spirova, R. and Bechev, B., ‘‘Modification of Lowry’s method for collagen concentration measurement’’, Biochem. Biophys. Method., 32(1): 33-43, (1996).

Fisher, D.A. and Laksmanan, J., ‘‘Metabolism and affects of epidermal growth factor and related growth factors in mammals’’, Endoc. Rev., 11(3): 418-422, (1990).

Dijke, P. and Iwata, K.K., ‘‘Growth factors for wound healing’’, Nature Biotecnology, 7: 793- 798, (1989).

Bhora, F.Y., Dunkin, B.J., Batzri, S., Aly, H.M., Bass, B.L., Sıdaway, A.N. and Harmon, J.W., ‘‘Effect of growth factors on celi proliferation and epithelization in human skin’’, J. Res. Surg., 59(2): 236-244, (1995).

Ekmekçi, A. and Erbaş, D., ‘‘Kanserin moleküler mekanizması. onkojenler ve büyüme faktörleri’’, 72 TDFD, Ankara, 45-78, (1991).

Tettamanti, G., Grimaldi, A., Rinaldi, L., Arnaboldi, F., Congiu, T., Valvassori, R. and Eguiler, M., ‘‘The multifunctional role of fibroblasts during wound healing in Hirudo medicinalis (Annelida, Hirudinea)’’, Biol. Cell., 96(6): 443-455, (2004).

Laato, M., Kahari, V.M., Niinikoski, J. and Vuorio, E., ‘‘Epidermal growth factor increases collagen production in granulation tissue by stimulation of fibroblast proliferation and not by activation of procollagen genes’’, Biochem. J., 247(2): 385-388, (1987).

Babül, A., Gönül, B., Özoğul, C., Dinçer, S., Erdoğan, D., Pınar, L. and Çelebi, N., ‘‘EGF accelerates mice skin wound healing’’, Pharm. Technol. Symp. (IPTS-96), Ankara, 9-11, (1996). [31] Erbaş, D., Güvendik, G., Gönül, B., ‘‘Submandibular bez ekstrelerinin serum çinko düzeylerine etkileri’’, Ankara Ecz. Fak. Derg., 17(1): 11-17, (1987).

Çelebi, N., Erden, N., Gönül, B. and Koz, M., ‘‘Effects of epidermal growth factor dosage forms on dermal wound strength in mice’’, J. Pharm. Pharmacol., 46: 386-387, (1994).

Yeler, H., Yücetaş, Ş., Yılmaz, D., Öztürk, M., Arıcı, S., ‘‘Epidermal büyüme faktörü (EGF)’nün diş çekim tarası üzerine etkisinin incelenmesi’’, Cumhuriyet Üniversitesi Diş Hekimliği Fakültesi Dergisi, 2(1): 25-31, (1999).

Klegerman, M.E. and Plotnıkoff. N.P., ‘‘Proteins as biological response modifiers in Klegerman’’, Groves, M.J. (Ed.), Pharmaceutical Biotecnology Interpharm, USA, 89-102, (1992).

Konturek, S.J., Dembınskı, A., Vvarzecha, Z., Brzozovvskı, T. and Gregor, H., ‘‘Role of epidermal growth factor in healing of chronic gastroduedonal ulcers in rats’’, Gastroenterology, 94: 1300-1307, (1988).

Laato, M., Niinikoshi, J., Gerdin, B. and Lebel, L., ‘‘Stimulation of wound healing by epidermal grown factor’’, Ann. Surg. J., 203(14): 379-381, (1986).

Patt, L.M. and Houck, J.C., ‘‘Role of polypeptide growth factors in normal and abnormal growth’’, Kidney Jnt., 23: 610-615, (1990).

Yerushalmı, N., Arad, A. and Margalit, R., ‘‘Molecular and cellular studies of hyaluronic acid-modified liposomes as bioadhesive carriers for topical drug delivery in wound healing’’, Arch. Biochem. Biophys., 313(2): 267-273, (1994).

Palanga, V., Eagstein, W.H.H., Bucalo, B., Harris, B. and Carson, P., ‘‘Topical use of human recombinant epidermal growth factor (h-egf) in venouk ulcers’’, J. Dermatol. Surg. Oncol., 18(7): 604-606, (1992).

Türkyılmaz, A., Çelebi, N., Gönül, B., ‘‘Epidermal büyüme faktörü (EGF)’nün yara iyileşmesindeki rolü’’, FABAD J. Pharm. Sci., 21:61-69, (1996).

Cellini, M., Baldi, A., Camarazza, N., De Fellici, G.P. and Gazzaniga, A., ‘‘Epidermal growth factor in the topical treatment of herpetic corneal ulcers’’, Ophthalmological, 208: 37-40, (1994).

Carpenter, G. and Cohen, S., ‘‘Epidermal growth factor’’, Ann. Rev. Biochem., 48: 193-216, (1979).

Ural, M., Koçak, A., Aksoy, A., ‘‘Yüz ve çene gelişimine etki eden faktörler’’, S.D.Ü. Diş Hekimliği Fakültesi Dergisi, 14(1): 41-44, (2007).

Hu, C.C., Sakakura, Y., Sasano, Y., Shum, L., Bringas, P. Jr., Werb 2, Slaukin, H.C., ‘‘Endogenous epidermal growth factor regulates the timing and pattern of embriyonic mouse molar toothmorphogenesis’’, Int. J. Dev. Biol., 36(4): 505-516, (1992).

Murray, R.K., Granner, D.K., Mayes, P.A. and Rodwell, W.W., Hücre Dışı Yatak, Harper’ın Biyokimyası, Nobel Tıp Kitabevi, İstanbul, 695- 700, (2004).

Gope, R., ‘‘The effects of epidermal growth factor and plateled derived growth factors on wound healing process’’, Indian J. Med. Res., 116: 201- 206, (2002).

Kim, Y.S., Lew, D.H., Tark, K.C., Rah, D.K. and Hong, J.P., ‘‘Effect of recombinant human epidermal growth factor against cutaneous scar formation in murine full-thickness wound healing’’, J. Korean Med. Sci., 25(4): 598-596, (2010).