Effect of Bacillus thuringiensis kurstaki on The Thaumetopoea pityocampa (Lepidoptera: Thaumetopoeidae), The Pine Processionary Caterpillar

Meltem UZUNHİSARCIKLI, Yusuf KALENDER, Zekiye SULUDERE
2.213 735

Abstract


In this study effects of Bacillus thuringiensis kurstaki (Btk) on midgut cells of Thaumetopoea pityocampa larvae was investigated by electron microscopy. 3 mg/l Btk was given with food. 1 h, 3 h and 6 h after Btk administration no pathological changes in the midgut epitelial cells were observed. 12 h after Btk administration swelling of mitochondria in midgut epitelial cells were observed. Formation of vacuoles in cytoplasm was seen after 24 h. After 48 h decreasing of microvilli of midgut epithelial cell’s and partial dissolving of cytoplasm, dissolving of nucleoplasm and chromatin clumps were observed. 60 h after Btk administration swelling and dissolving of mitochondria in midgut epithelial cells were detected. 96 h after Btk administration a membrane-body and dissolving in the cytoplasm clumps in midgut cells of T. pityocampa larvae were occurred. Present study shows that Btk is effective to T. pityocampa larvae.


Keywords


Thaumetopoea pityocampa, Bacillus thuringiensis kurstaki, midgut, ultrastructure, transmission electron microscope

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